Application notes

Monitoring protein aggregation in injectable vaccines syringes: a new direct & contactless technique

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Aggregation of proteins and active principle ingredients (API) in injectable biopharmaceutical products remains a major concern impacting the stability and usability of a product. This mechanism is still not well understood. The Dynamic Light Scattering (DLS) in situ monitoring of the denaturation and degradation process of therapeutic proteins during production and storage can be a key competitive advantage for manufacturers and researchers.

NIR Dynamic Light Scattering for Quantum Dots Size Measurement

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Since QD size (usually,≤30 nm) is the key to their optical properties, Dynamic Light Scattering (DLS) presents itself as an interesting candidate for such characterization, since it enables a representative size measurement of small nanoparticles (NPs) in suspensions. To avoid the difficulties due the QDs absorption properties, replacement of the classical 635-nanometers laser by a near-infrared (NIR) 780-nanometers laser in the DLS apparatus made such a measurement possible. This study makes evidence that it is possible to extend the range of DLS uses for fluorescent particles with strong absorbance in the visible, making it a promising characterization technique for fluorophore-based system, especially in the biomedical field.